IL-17 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in-vitro enzyme-linked immunosorbent assay to quantitatively measure human IL-17 in supernatant plasma and cell culture. This assay uses an IL-17 specific antibody coated on a 96-well plate for Human IL. Standards and samples are piped into the wells, and the immobilized antibody binds IL-17 present in a sample to the wells. The IL-17 ELISA Kit is designed to detect native not recombinant. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
IL-17 ELISA kits has the below components:
Microplate is a critical part of IL-17 ELISA. The microplates have the capability to generate valuable, information-rich data that bring great insights into discovery screening. IL-17 ELISA microplates address the most common screening challenges. It has been engineered to deliver the highest quality data for high throughput screening as well as imaging applications. The IL-17 ELISA microplate meets the below mentioned standards:
a) IL-17 ELISA microplate footprint measurements comply with the SBS industry standard, ensuring compliance with microplate-based instrumentation
b) IL-17 ELISA has controlled microplate planarity to ensure excellent data collection and image collection
c) IL-17 ELISA has custom microplate services including barcoding, custom packaging and plate surface coatings to suit the application.
d) IL-17 ELISA microplate is afull range of validated reagents and instruments to compliment microplates
By using IL-17 ELISA microplates specifically designed for high throughput screening and imaging applications, you can be confident that you will obtain high quality results that will provide more information-rich data and experience fewer re-screens that will reduce your overall screening costs.
The intended use of the Human IL-17A RSG ELISA Lyophilized Standard is a standard for Human IL-17A ELISA. Typically, the standard is used in a series of 7 duplicate dilutions of the highest standard.
Sample/Standard Dilution Buffer
Determine the concentration of the target protein in the test sample to ensure the target protein concentration falls within the optimal detection range of the IL-17 ELISA kit
IL-17 ELISA Biotin conjugated anti-IL-15 polyclonal antibody is used as detection antibodies. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates were washed away with wash buffer.
Antibody Dilution Buffer
This buffer is used to dilute a range of sample types or for the dilution of detection antibodies. It reduces cross-reactivity, non-specific binding, and matrix effects.
IL-17 ELISA fluorescent conjugates of streptavidin are used to detect biotinylated biomolecules such as primary and secondary antibodies, ligands and toxins. is most commonly used and it is non-glycosylated and exhibits low levels of nonspecific binding. Enzyme-labeled conjugates of streptavidin and avidin biotin-binding proteins, including streptavidin horseradish peroxidase (HRP) and streptavidin alkaline phosphatase (AP), provide tools needed for IL-17 ELISA kit.
In addition to this high affinity, the Biotin/ Streptavidin System can be effectively exploited because Streptavidin has four binding sites for biotin and most proteins (including antibodies and enzymes) can be conjugated with several molecules of biotin. These aspects provide the potential for macromolecular complexes to be formed between Streptavidin and biotinylated enzymes.
SABC Dilution Buffer
IL-17 ELISA dilution buffers are designed to avoid immunoassay interference which can produce false positive or bad results. This could be due to cross-reactions or matrix effects when you have significant background noise or false positives. To solve these issues, it is recommended that adequate dilution buffer be used.
IL-17 ELISA kit TMB substrates is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-17 amount of sample captured in plate.
The adding order of stop solution should be as the same as the substrate solution. terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm.
Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper.
standard adhesive plate seals and lids are used to prevent well to well cross contamination Applications.